To observe the expression of H-ras p21 and p53 in cancer cells with clinical data and different pathologic findings of lung cancer, immuohistochemical studies were carried out by using avidin-biotin complex immuoperoxidase method in
paraffin-embedded
tissue sections from 27 cases.
The studies consisted of 18 cases of bronchogenic squamous cell carcinoma, including 4 cases of well differentiated type and 14 cases of moderately differentiated type, 7 cases of primary adenocarcinoma, and 2 cases of metastatic carcinoma,
including 1
cases of metastatic denocarcinoma from stomach and 1 cases of metastatic transitional cell carcinoma from urinary bladder.
@ES The results were as follows:
@EN 1. Positive reactions of H-ras p21 in carcinoma were observed in 72% of the squamous cell carcinomas and 87 T of the adenocarcinomas.
2. Positive reactions of p53 in the carcinoma were observed in 34 % of the squamous cell carcinomas and 62.5% of the adenocarcinomas.
3. Correlation between patient's age and oncogene expression was statistically not significant.
4. Correlation between tumor size and oncogene expression was statistically not significant.
5. Correlation between stage and oncogene expression was statistically not significant.
6. Correlation between tumor size and stage was statistically significant (Kendall's tau-b Coefficients=0.4405, P<0.05).
7. Correlation between H-ras p21 and p53 expression was statistically significant (kendall's tau-b Coefficients=0.6123, P<0.01).
8. Therefore, authors suggested that H-ras p21 and p53 expression was involved in the progression of lung cancer.
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